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KMID : 1044820170470060402
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Journal of Periodontal & Implant Science
2017 Volume.47 No. 6 p.402 ~ p.415
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Evaluation of the periodontal regenerative properties of patterned human periodontal ligament stem cell sheets
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Kim Joong-Hyun
Ko Seok-Yeong
Lee Justin-Ho
Kim Deok-Ho
Yun Jeong-Ho
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Abstract
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Purpose: The aim of this study was to determine the effects of patterned human periodontal ligament stem cell (hPDLSC) sheets fabricated using a thermoresponsive substratum.
Methods: In this study, we fabricated patterned hPDLSC sheets using nanotopographical cues to modulate the alignment of the cell sheet.
Results: The hPDLSCs showed rapid monolayer formation on various surface pattern widths. Compared to cell sheets grown on flat surfaces, there were no significant differences in cell attachment and growth on the nanopatterned substratum. However, the patterned hPDLSC sheets showed higher periodontal ligamentogenesis-related gene expression in early stages than the unpatterned cell sheets.
Conclusions: This experiment confirmed that patterned cell sheets provide flexibility in designing hPDLSC sheets, and that these stem cell sheets may be candidates for application in periodontal regenerative therapy.
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KEYWORD
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Extracellular matrix, Periodontal ligament, Regeneration, Stem cells, Tissue engineering
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